Mahajan, Ritu ; Singh, Randhir (1989) Properties of pyrophosphate:fructose-6-phosphate phosphotransferase from endosperm of developing wheat (Triticum aestivum L.) grains Plant Physiology, 91 (1). pp. 421-426. ISSN 0032-0889
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Official URL: http://www.plantphysiol.org/content/91/1/421.short
Related URL: http://dx.doi.org/10.1104/pp.91.1.421
Abstract
Pyrophosphate:fructose-6-phosphate phosphotransferase (PFP, EC 2.7.1.90) from endosperm of developing wheat (Triticum aestivum L.) grains was purified to apparent homogeneity with about 52% recovery using ammonium sulfate fractionation, ion exchange chromatography on DEAE-cellulose and gel filtration through Sepharose-CL-6B. The purified enzyme, having a molecular weight of about 170,000, was a dimer with subunit molecular weights of 90,000 and 80,000, respectively. The enzyme exhibited maximum activity at pH 7.5 and was highly specific for pyrophosphate (PPi). None of the nucleoside mono-, di- or triphosphate could replace PPi as a source of energy and inorganic phosphate (Pi). Similarly, the enzyme was highly specific for fructose-6- phosphate. It had a requirement for Mg2+ and exhibited hyperbolic kinetics with all substrates including Mg2+. Km values as determined by Lineweaver-Burk plots were 322, 31, 139, and 129 micromolar, respectively, for fructose-6-phosphate, PPi, fructose- 1,6-bisphosphate and Pi. Kinetic constants were determined in the presence of fructose-2,6-bisphosphate, which stimulated activity about 20-fold and increased the affinity of the enzyme for its substrates. Initial velocity studies indicated kinetic mechanism to be sequential. At saturating concentrations of fructose-2,6- bisphosphate (1 micromolar), Pi strongly Inhibited PFP; the inhibition being mixed with respect to both fructose-6-phosphate and PPi, with K, values of 0.78 and 1.2 millimolar, respectively. The inhibition pattem further confirmed the mechanism to be sequential with random binding of the substrates. Probable role of PFP in endosperm of developing wheat grains (sink tissues) is discussed.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society of Plant Biologists. |
ID Code: | 50066 |
Deposited On: | 21 Jul 2011 14:41 |
Last Modified: | 21 Jul 2011 14:41 |
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