Srivastava, Toolika ; Das, Sandip ; Sopory, Sudhir Kumar ; Srivastava, P. S. (2009) A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens Physiology and Molecular Biology of Plants, 15 (1). pp. 93-98. ISSN 0971-5894
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Official URL: http://www.springerlink.com/content/946t8762j880h3...
Related URL: http://dx.doi.org/10.1007/s12298-009-0010-1
Abstract
Proliferation of axillary shoot buds and multiple shoot formation in Catharanthus roseus was obtained in 96 % explants on MS medium (3% sucrose) containing NAA+BA. 2,4-D induced callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell concentration, duration of co-cultivation and acetosyringone concentration influenced transformation efficiency. Under optimal co-cultivation conditions, 98 % of the explants showed GUS expression. PCR based amplification of the transformed and subsequently selected callus tissue indicated the presence of uidA, Gly I and nptII genes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Catharanthus roseus; Micropropagation; RAPD; Co-cultivation; GUS; GlyI; Agrobacterium tumefaciens; Transformation |
| ID Code: | 49967 |
| Deposited On: | 21 Jul 2011 09:52 |
| Last Modified: | 21 Jul 2011 09:52 |
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