Singla-Pareek, Sneh L. ; Yadav, Sudesh Kumar ; Pareek, Ashwani ; Reddy, M. K. ; Sopory, S. K. (2008) Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II Transgenic Research, 17 (2). pp. 171-180. ISSN 0962-8819
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Official URL: http://www.springerlink.com/content/9630863213q8j1...
Related URL: http://dx.doi.org/10.1007/s11248-007-9082-2
Abstract
Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Functional Validation; Glyoxalase II Overexpression; Methylglyoxal; Rice Transgenics; Salinity Tolerance |
| ID Code: | 49935 |
| Deposited On: | 21 Jul 2011 09:51 |
| Last Modified: | 21 Jul 2011 09:51 |
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