Deswal, Renu ; Sopory, Sudhir K. (1991) Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea FEBS Letters, 282 (2). pp. 277-280. ISSN 0014-5793
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0014-5793(91)80494-N
Abstract
The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B, Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Glyoxalase I; Enzyme Purification; Methylglyoxal; Glutathione; S-hexyl Glutathione |
| ID Code: | 49918 |
| Deposited On: | 21 Jul 2011 09:16 |
| Last Modified: | 21 Jul 2011 09:16 |
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