Kumar, Polumetla A. ; Mandaokar, Ajin ; Sreenivasu, Karra ; Chakrabarti, Swarup K. ; Bisaria, Suman ; Sharma, Surat R. ; Kaur, Sarvjeet ; Sharma, Rameshwar P. (1998) Insect-resistant transgenic brinjal plants Molecular Breeding, 4 (1). pp. 33-37. ISSN 1380-3743
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Official URL: http://www.springerlink.com/content/q5l57318283g1v...
Related URL: http://dx.doi.org/10.1023/A:1009694016179
Abstract
A synthetic cry1Ab gene coding for an insecticidal crystal protein (ICP) of Bacillus thuringiensis (Bt) was transferred to brinjal (eggplant) by cocultivating cotyledonary explants with Agrobacterium tumefaciens. Transformant plants resistant to kanamycin were regenerated. Hybridization experiments demonstrated gene integration and mRNA expression. Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants. The expression resulted in a significant insecticidal activity of transgenic brinjal fruits against the larvae of fruit borer (Leucinodes orbonalis). The results also demonstrated that a synthetic gene based on monocot codon usage can be expressed in dicotyledonous plants for insect control.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Bacillus thuringiensis; Insecticidal Crystal Protein Gene; cry1Ab; Brinjal; Fruit Borer; Leucinodes orbonalis; Transgenic Plants |
| ID Code: | 49381 |
| Deposited On: | 20 Jul 2011 05:54 |
| Last Modified: | 20 Jul 2011 05:54 |
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