Chakrabarti, S. K. ; Mandaokar, A. D. ; Shukla, A. ; Pattanayak, D. ; Naik, P. S. ; Sharma, R. P. ; Kumar, P. A. (2000) Bacillus thuringiensis cry1Ab gene confers resistance to potato against Helicoverpa armigera (Hubner) Potato Research, 43 (2). pp. 143-152. ISSN 0014-3065
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Official URL: http://www.springerlink.com/content/g45272w22673h6...
Related URL: http://dx.doi.org/10.1007/BF02357955
Abstract
Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) of Bacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays on the leaves of transgenic plants showed considerable protection against the larvae of H. armigera in terms of leaf area consumed and larval weight reduction.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Transgenic Plants; Solanum tuberosum L. |
| ID Code: | 49379 |
| Deposited On: | 20 Jul 2011 06:08 |
| Last Modified: | 20 Jul 2011 06:08 |
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