Mandaokar, A. D. ; Goyal, R. K. ; Shukla, A. ; Bisaria, S. ; Bhalla, R. ; Reddy, V. S. ; Chaurasia, A. ; Sharma, R. P. ; Altosaar, I. ; Ananda Kumar, P. (2000) Transgenic tomato plants resistant to fruit borer (Helicoverpa armigera Hubner) Crop Protection, 19 (5). pp. 307-312. ISSN 0261-2194
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0261-2194(00)00022-3
Abstract
A synthetic cry1Ac gene coding for an insecticidal crystal protein (ICP) of Bacillus thuringiensis (Bt) was transferred to tomato by cocultivating cotyledonary explants with Agrobacterium tumefaciens. Transformant plants resistant to kanamycin were regenerated. Hybridization experiments demonstrated gene integration and gene copy number in the transgenic plants. Double-antibody sandwich ELISA analysis revealed high levels of Bt ICP expression in the leaves of transgenic plants. The expression resulted in a high level of protection of transgenic plant leaves and fruits against the larvae of tomato fruit borer (Helicoverpa armigera). Limited field trial of the transgenic plants (T1 generation) confirmed the high levels of insect protection.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Bacillus thuringiensis; Insecticidal Protein Gene; cry1Ac; Tomato; Fruit Borer; Helicoverpa armigera; Transgenic Plants |
| ID Code: | 49378 |
| Deposited On: | 20 Jul 2011 06:08 |
| Last Modified: | 20 Jul 2011 06:08 |
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