Ghosh, Arundhati ; Joshi, Vishwas D. ; Shaila, M. S. (1995) Characterization of an in vitro transcription system from rinderpest virus Veterinary Microbiology, 44 (2-4). pp. 165-173. ISSN 0378-1135
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0378-1135(95)00009-Y
Abstract
An in vitro transcription system for rinderpest virus (RPV) is described. Ribonucleoprotein complexes isolated from RPV-infected Vero cells, human lung carcinoma cells, or detergent-disrupted purified virions synthesized authentic RPV mRNAs for the N, P, M, F and H genes as identified by dot blot hybridization analysis with individual cDNA clones. The relative abundance of the mRNAs synthesized in vitro decreased from the 3' end of the genome to the 5' end, very similar to that observed with measles virus transcription in vitro. The transcription by purified virions was stimulated three-fold by the addition of infected human lung carcinoma cell lysate, demonstrating the involvement of host factor(s) in mRNA synthesis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Rinderpest Virus; RNP Complex; Transcription in Vitro; Stimulation by Host Factor(s) |
| ID Code: | 49278 |
| Deposited On: | 19 Jul 2011 13:59 |
| Last Modified: | 19 Jul 2011 13:59 |
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