Udo, Edet E. ; Farook, Vidya S. ; Mokadas, Eiman M. ; Jacob, Latha E. ; Sanyal, Suhas C. (1999) Molecular fingerprinting of mupirocin-resistant methicillin-resistant Staphylococcus aureus from a burn unit International Journal of Infectious Diseases, 3 (2). pp. 82-87. ISSN 1201-9712
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S12019...
Abstract
Objectives: To characterize mupirocin-resistant methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients in a burn unit by pulsed-field gel electrophoresis and plasmid contents. Methods: A total of 53 methicillin-resistant S. aureus, consisting of 48 mupirocin-resistant and 5 mupirocin-susceptible MRSA were compared by plasmid content and pulsed-field gel electrophoresis of Sma I digested genomic DNA. Results: Of the 48 mupirocin-resistant isolates, 39 expressed high-level, and 9 expressed low-level mupirocin resistance. Plasmids were detected in all of the 53 isolates; however, only the high-level mupirocin-resistant isolates contained a 38 kb-conjugative plasmid that encoded high-level mupirocin resistance. Pulsed-field gel electrophoresis divided the isolates into four patterns designated types I to IV Forty-three isolates consisting of 34 high-level, 5 low-level mupirocin-resistant and 4 mupirocin-susceptible isolates defined the type-I pattern. Eight isolates, five high-level and three low-level mupirocin-resistant isolates had the type-II pulsed-field pattern. The type-III and type-IV pulsed-field patterns consisted of a single isolate each. The typed and type-II pulsed-field patterns were related and only differed by four Sma I bands. Conclusions: Results of typing the mupirocin-resistant MRSA that closely related MRSA clones previously circulating in the unit had acquired a high-level mupirocin-resistant plasmid, and spread aided by mupirocin use.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Burn Unit; Methicillin-resistant S. aureus; Mupirocin-resistance; Pulsed-field Gel Electrophoresis |
ID Code: | 49251 |
Deposited On: | 20 Jul 2011 06:37 |
Last Modified: | 18 May 2016 04:02 |
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