Gayathri Devi, B. ; Ramakrishnan, T. ; Gopinathan, K. P. (1974) Studies on an enzyme reacting with isoniazid from Mycobacterium tuberculosis H37Rv Proceedings of the Indian Academy of Sciences - Plant Sciences, 80 (5). pp. 240-252. ISSN 0253-410X
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Official URL: http://www.ias.ac.in/jarch/procb/80/240-252.pdf
Related URL: http://dx.doi.org/10.1007/BF03050671
Abstract
Mycobacterium tuberculosis H37Rv possesses an enzyme (referred to as 'Y enzyme') which catalyses in the presence of INH and NAD, the formation of a product, which turns yellow on acidification. The requirements for the reaction, such as enzyme concentration, INH concentration, etc., have been standardized. The substrate specificity of the enzyme with respect to INH and NAD has been determined. The reaction is specific for the INH-sensitive strain and is totally absent in INH-resistant strains. Furthermore, the 'Y enzyme' shows some characteristic features of a peroxidase in its requirement for oxygen and sensitivity to inhibition by various reagents. The requirements of this enzyme which is involved in the action of isoniazid in M. tuberculosis H37Rv is described for the first time.
Item Type: | Article |
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Source: | Copyright of this article belongs to Indian Academy of Sciences. |
ID Code: | 49225 |
Deposited On: | 19 Jul 2011 11:54 |
Last Modified: | 18 May 2016 04:01 |
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