Enzymes in Candida albicans II. Tricarboxylic acid cycle and related enzymes

Abstract

Evidence is presented to show the operation of the tricarboxylic acid cycle in Candida albicans, by studies with whole cells, cell-free preparations, and by the demonstration of most of the enzymes involved in the cycle. Cell-free extracts contained the following enzymes: condensing enzyme; aconitase; isocitric, α-ketoglutaric, succinic, and malic dehydrogenases; malic enzyme; fumarase; reduced diphosphopyridine nucleotide (DPNH) oxidase; DPNH-cytochrome c reductase; reduced triphosphopyridine nucleotide (TPNH) cytochrome c reductase; and diaphorase. Pyruvic dehydrogenase, TPNH oxidase, and transhydrogenase activities could not be detected under the test conditions.