Rao, D. N. Ramakrishna ; Mahadevan, S. ; Balaram, P. (1978) Structural studies of the hen's egg lipovitellins. NMR and fluorescence investigations Chemistry and Physics of Lipids, 22 (2). pp. 105-113. ISSN 0009-3084
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000930...
Related URL: http://dx.doi.org/10.1016/0009-3084(78)90035-X
Abstract
The pH dependent reversible association-dissociation reaction of α- and β-lipovitellins from egg yolk has been studied by 1H NMR and fluorescence probe methods. Increased mobility of the choline methyl groups has been demonstrated on dissociation. The lipid methylene resonance of β-lipovitellin shows clear doublet character suggesting that the fatty acid chains exist in distinct environments. The high field component increases with temperature but is suppressed on treatment with pronase, suggesting a significant role for proteins in maintaining the differences in lipid environments. 1-Anilino-8-naphthalene sulfonate has been shown to bind less effectively to the monomeric lipovitellins. This is in agreement with earlier results suggesting that dissociation may be accompanied by increased hydration and conformational changes.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
ID Code: | 4904 |
Deposited On: | 18 Oct 2010 06:16 |
Last Modified: | 16 May 2016 15:29 |
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