Novel K540N mutation in Plasmodium falciparum dihydropteroate synthetase confers a lower level of sulfa drug resistance than does a K540E mutation

Lumb, Vanshika ; Sharma, Yagya D. (2011) Novel K540N mutation in Plasmodium falciparum dihydropteroate synthetase confers a lower level of sulfa drug resistance than does a K540E mutation Antimicrobial Agents and Chemotherapy, 55 (5). pp. 2481-2482. ISSN 0066-4804

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Official URL: http://aac.asm.org/cgi/content/abstract/55/5/2481

Related URL: http://dx.doi.org/10.1128/AAC.01394-10

Abstract

Sulfadoxine (SDX) and sulfamethoxazole (SMX) each inhibit the Plasmodium falciparum dihydropteroate synthetase (PfDHPS), and certain point mutations in this enzyme yield the drug-resistant parasite. Using a simple Escherichia coli model system, we describe here the effect of the recently reported novel K540N mutation in PfDHPS on the level of SDX/SMX resistance. The survival rate of the transformed E. coli (DHPS-deficient strain) under different SDX or SMX concentrations revealed that the K540N mutation confers a lower level of drug resistance than its contemporary K540E mutation. Further, SMX was more effective than SDX in the E. coli system.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:48678
Deposited On:15 Jul 2011 08:00
Last Modified:15 Jul 2011 08:00

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