In vitro synthesis of oligosaccharides by three "fruit rot" fungi

Abstract

The influence of component monosaccharide sugars on thein vitro synthesis of oligosaccharides by the three fungi,viz., Macrophoma allahabadensis Kapoor and Tandon, Curvularia tuberculata Jain and Drechslera australiense (Bugn.) Subram. and Jain, has been studied. The former two organisms were isolated from the diseased fruits of guava and the last from tomato. The present investigations have shown that the persistence of oligosaccharides could be altered by extra supply of the component sugars in the medium. When maltose was supplied at the rate of 10 g./litre in the medium an oligosaccharide I (R_f 0.18) was synthesized by all the three organisms. An addition of 5 g. glucose to a maltose medium induced the synthesis of an additional oligosaccharide II (R_f0.30). Thus oligosaccharide (R_f 0.30) was produced only in the culture filtrate of Macrophoma allahabadensis and Curvularia tuberculata. The addition of D-glucose or D-fructose to a sucrose medium showed no effect on the synthesis of oligosaccharide. The nature of synthesized oligosaccharides was studied partially after subjecting them to total and partial hydrolysis and results have been recorded.