A new method for the generation of patterned protein films by encapsulation in arrays of thermally evaporated lipids

Gole, Anand ; Sastry, Murali (2001) A new method for the generation of patterned protein films by encapsulation in arrays of thermally evaporated lipids Biotechnology and Bioengineering, 74 (2). pp. 172-178. ISSN 0006-3592

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/bit.110...

Related URL: http://dx.doi.org/10.1002/bit.1107

Abstract

In this article we demonstrate a versatile method for the generation of patterned protein films by encapsulation in arrays of the lipids, octadecylamine (ODA, cationic), and arachidic acid (AA, anionic). A simple 2 × 2 array of ODA and AA was vacuum deposited on different substrates using appropriate masks. Thereafter, the enzymes pepsin and fungal protease as well as the heme-proteins cytochrome c and hemoglobin were encapsulated in the different elements of the array by sequential immersion (combined with judicious masking) of the array elements in the different protein solutions. The proteins are incorporated into the lipid elements by electrostatic interaction between charged amino acid residues on the protein surface and charged functional groups in the lipid matrix. This procedure leads to spatially distinct regions of the different proteins on one substrate and shows promise for single-chip multianalyte immunoassay/multiplex, high-throughput biosensor and catalysis applications. Fourier transform infrared spectroscopy (FTIR) was used to monitor the incorporation of the proteins in the different elements of the array as well as to ascertain whether intermixing of the proteins in a particular array element had occurred. The heme-protein composite regions were further characterized using UV-VIS spectroscopy.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Patterned Protein Films; Encapsulation; Lipids; FTIR; Enzymes; Biosensors
ID Code:47232
Deposited On:07 Jul 2011 04:54
Last Modified:07 Jul 2011 04:54

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