Phadtare, Sumant ; Vinod, V. P. ; Wadgaonkar, Prakash P. ; Rao, Mala ; Sastry, Murali (2004) Free-standing nanogold membranes as scaffolds for enzyme immobilization Langmuir, 20 (9). pp. 3717-3723. ISSN 0743-7463
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Official URL: http://pubs.acs.org/doi/abs/10.1021/la035870j
Related URL: http://dx.doi.org/10.1021/la035870j
Abstract
We demonstrate herein the formation of a free-standing gold nanoparticle membrane and its use in the immobilization of the enzyme, pepsin. The nanogold membrane is synthesized by the spontaneous reduction of aqueous chloroaurate ions at the liquid-liquid interface by the bifunctional molecule bis(2-(4-aminophenoxy)ethyl) ether (DAEE) taken in chloroform. This process results in the formation of a robust, malleable free-standing nanogold membrane consisting of gold nanoparticles embedded in a polymeric background. Recognizing that gold nanoparticles are excellent candidates for immobilization of enzymes, we have immobilized pepsin on the nanogold membrane, leading to a new class of biocatalyst. A highlight of the new pepsin-nanogold biocatalyst is the ease with which separation from the reaction medium may be achieved. The catalytic activity of pepsin in the bioconjugate was comparable to that of the free enzyme in solution. The pepsin-nanogold membrane bioconjugate material exhibited excellent biocatalytic activity over 10 successive reuse cycles as well as enhanced pH, temperature, and temporal stability.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Chemical Society. |
ID Code: | 47108 |
Deposited On: | 06 Jul 2011 14:18 |
Last Modified: | 06 Jul 2011 14:18 |
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