Banded krait minor satellite (Bkm) contains sex and species-specific repetitive DNA

Panicker, S. G. ; Singh, L. (1994) Banded krait minor satellite (Bkm) contains sex and species-specific repetitive DNA Chromosoma, 103 (1). pp. 40-45. ISSN 0009-5915

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Official URL: http://www.springerlink.com/content/x1n3670076857l...

Related URL: http://dx.doi.org/10.1007/BF00364724

Abstract

A novel class of repetitive DNA was isolated from a Bkm DNA library by exclusion hybridization. This sequence was mapped to the short arm of the W chromosome of banded krait, Bungarus fasciatus. Southern blot hybridization showed that these sequences are sex and species specific. Sequence analysis of a 206 bp long clone, BR87, revealed the presence of a tandem array of two internal repeat units of 18-19 bp alternating with each other with a gap of 1,2 or 3 nucleotides. To our knowledge, this is the first report of an exclusively W chromosome-and species-specific repeat isolated from any reptile. The functional significance of this sequence based on its organisation is discussed.

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