Vijayasarathy, S. ; Shivaji, S. ; Balaram, P. (1982) Bull sperm plasma and acrosomal membranes: fluorescence studies of lipid phase fluidity Biochemical and Biophysical Research Communications, 108 (2). pp. 585-591. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(82)90868-3
Abstract
Bull sperm plasma and outer acrosomal membranes have been isolated by discontinuous sucrose density gradient centrifugation and Ca2+-ATPase activity has been determined for both the membranes. Pyrene excimer fluorescence and diphenylhexatriene fluorescence polarization studies show that the lipid phase of the sperm plasma membranes is more fluid than the lipids of the outer acrosomal membranes. Approximately, a three fold increase in the cholesterol content has been found in the outer acrosomal membranes as compared to that in the plasma membranes. HEPES, N-2-hydroxyethyl-piperazine-N′-2-ethane sulfonic acid; DPH, 1,6-Diphenyl-1,3-5-hexatriene .
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 4670 |
| Deposited On: | 18 Oct 2010 07:09 |
| Last Modified: | 16 May 2011 08:54 |
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