Geetha, S. ; Shetty, Sudheer A. ; Shekar Shetty, H. ; Prakash, H. S. (1998) Sclerospora graminicola- and arachidonic acid-induced autofluorescence in downy mildew resistant and susceptible genotypes of pearl millet Annals of Applied Biology, 133 (2). pp. 219-226. ISSN 0003-4746
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1744-...
Related URL: http://dx.doi.org/10.1111/j.1744-7348.1998.tb05822.x
Abstract
Autofluorescence of downy mildew resistant and susceptible cells of pearl millet seedlings undergoing hypersensitive reaction (HR) upon Sclerospora graminicola-inoculation and arachidonic acid (AA)-treatment was studied. Two-day-old seedlings of a highly resistant (IP 18296) and a highly susceptible (23D2B) genotype of pearl millet were either inoculated with zoospore suspension of S. graminicola or treated with AA for 24 h. The coleoptiles with hypersensitive necrotic spots were processed by the standard procedure, and the tissues were subjected to fluorescence microscopy. A differential accumulation of autofluor-escent compounds in resistant and susceptible pearl millet genotypes was observed with most accumulation occurring in resistant cells treated with AA. The variation in the degree of fluorescence and the spatial accumulation of autofluorescent compounds among the two inoculated/treated genotypes is discussed.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Association of Applied Biologists. |
| Keywords: | Pearl Millet; Downy Mildew; Autofluorescence; Resistant; susceptible; Arachidonic Acid |
| ID Code: | 46693 |
| Deposited On: | 06 Jul 2011 07:11 |
| Last Modified: | 06 Jul 2011 07:11 |
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