Kumar, Vasanthi U. ; Meera, M. S. ; Shishupala, S. ; Shetty, H. S. (1993) Quantification of Sclerospora graminicola in tissues of Pennisetum glaucum using an enzyme-linked immunosorbent assay Plant Pathology, 42 (2). pp. 251-255. ISSN 0032-0862
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...
Related URL: http://dx.doi.org/10.1111/j.1365-3059.1993.tb01497.x
Abstract
Antibodies raised against whole sporangia of Sclerospora graminicola reacted specifically with sporangia and mycelium of this fungus. Agglutination studies localized the antigen on the cell-wall surfaces. Using this antibody, an indirect enzyme-linked immunosorbent assay (ELISA) with a biotin-avidin amplification system was developed for the quantification of S. graminicola in the host tissues of Pennisetum glaucum. This sensitive assay system, which could detect 3.1-5.5 μg dry weight of fungal biomass, was used to monitor the increase in fungal biomass during disease progression.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| ID Code: | 46670 |
| Deposited On: | 06 Jul 2011 07:09 |
| Last Modified: | 06 Jul 2011 07:09 |
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