Saidapur, S. K. ; Nadkarni, V. B. (1975) Histochemical localization of Δ5-3β -hydroxysteroid dehydrogenase, glucose-6-phosphate dehydrogenase, and NADH- and NADPH-diaphorase activities in the testis of Rana hexadactyla and Cacopus systoma The Histochemical Journal, 7 (6). pp. 557-561. ISSN 0018-2214
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Official URL: http://www.springerlink.com/content/g7r4j130057t22...
Related URL: http://dx.doi.org/10.1007/BF01003793
Abstract
The distribution of Δ5-3β -hydroxysteroid dehydrogenase (Δ 5-3β-HSDH), glucose-6-phosphate dehydrogenase (G6PDH), NADH- and NADPH-diaphorases were localized histochemically in the testis of Rana hexadactyla and Cacopus systoma. Dehydroepiandrosterone (DHEA) and pregnenolone were used as the substrates for the demonstration of Δ5-3β -HSDH activity which occurs mainly in the interstitial Leydig cells and also in the columnar Sertoli cells. Both G6PDH and the NADPH-diaphorase show a distribution similar to that of Δ5-3β -HSDH but give intense reaction, whereas the distribution of NADH-diaphorase is ubiquitous. It is concluded that the Leydig cells form the principal site and the Sertoli cells an additional site of steroid biosynthesis in the testis of both R. hexadactyla and C. systoma.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| ID Code: | 45303 |
| Deposited On: | 25 Jun 2011 15:01 |
| Last Modified: | 27 Jun 2011 04:18 |
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