Jawali, Narendra ; Sainis, Jayashree Krishna ; Sane, Prafullachandra Vishnu (1978) Hydroxylamine inhibition of the nitrate reductase complex from Amaranthus Phytochemistry, 17 (9). pp. 1527-1530. ISSN 0031-9422
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0031-9422(00)94635-1
Abstract
Nitrate reductase from Amaranthus viridis is similar to nitrate reductase from other plant sources. NH2OH inhibits nitrate reduction from NADH by the nitrate reductase complex, but it does not inhibit either the NADH-dehydrogenase activity or nitrate reduction from reduced flavin mononucleotides. The inhibition observed was non-competitive with nitrate when the enzyme was pre-incubated with NH2OH and NADH, and competitive with nitrate without pre-incubation. The Ki values for NH2OH were 5 μM and 30 μM with or without pre-incubation respectively.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Amaranthus viridis; Amaranthaceae; Nitrate Reductase Complex; Hydroxylamine; Inhibition Kinetics |
| ID Code: | 45146 |
| Deposited On: | 25 Jun 2011 05:48 |
| Last Modified: | 25 Jun 2011 05:48 |
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