Borah, Babul ; Roy, Siddhartha ; Zon, Gerald ; Cohen, Jack S. (1985) Unique pyrimidine 2D-COSY aromatic cross-peaks as monitors of pyrimidine environments and mobility in oligo- and polynucleotides Biochemical and Biophysical Research Communications, 133 (2). pp. 380-388. ISSN 0006-291X
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0006-291X(85)90917-9
Abstract
Only cytosine contains two adjacent aromatic protons that give rise to cross-peaks in the aromatic region of 2D-COSY spectra of oligodeoxynucleotides. In two GC-containing sequences several such cross-peaks were resolved. The intensity of these cross-peaks is a sensitive monitor of local mobility, and upon the addition of the intercalating drug daunomycin selective intensity losses were observed, indicating binding to specific GC base pairs. We have also monitored the 2D-COSY cross-peaks from mobile pyrimidine bases in tRNA(Phe) as a function of temperature.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | 2D-COSY; Two-dimensional Proton J-correlated NMR Spectroscopy; DMT; 5'-Dimethoxytrityl; NOE; Nuclear Overhauser Effect |
| ID Code: | 43171 |
| Deposited On: | 10 Jun 2011 07:16 |
| Last Modified: | 10 Jun 2011 07:16 |
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