Roy, Siddhartha (1983) A continuous spectrophotometric assay for Escherichia coli alanyl-transfer RNA synthetase Analytical Biochemistry, 133 (2). pp. 292-295. ISSN 0003-2697
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0003-2697(83)90086-6
Abstract
A new continuous spectrophotometric assay is demonstrated for Escherichia coli alanyl-tRNA synthetase. It involves β-γ adenylyl imidophosphate as a substitute for ATP in the pyrophosphate exchange reaction. The net conversion of β-γ adenylyl imidophosphate to ATP can be linked to NADP reduction by hexokinase and glucose-6-P dehydrogenase catalyzed reactions, which can be monitored at 340 nm. This assay can be extended to other aminoacyl-tRNA synthetases which can use β-γ nonhydrolyzable analogs of ATP as an ATP substitute.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | tRNA Synthetase; Spectrophotometric; Continuous Assay; AMPPNP; Pyrophosphate Exchange |
| ID Code: | 43166 |
| Deposited On: | 10 Jun 2011 07:11 |
| Last Modified: | 10 Jun 2011 07:11 |
Repository Staff Only: item control page
