Mandal, Amit Kumar ; Samaddar, Soma ; Banerjee, Rajat ; Lahiri, Simanti ; Bhattacharyya, Anusree ; Roy, Siddhartha (2003) Glutamate counteracts the denaturing effect of urea through its effect on the denatured state Journal of Biological Chemistry, 278 (38). pp. 36077-36084. ISSN 0021-9258
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Official URL: http://www.jbc.org/content/278/38/36077.short
Related URL: http://dx.doi.org/10.1074/jbc.M211207200
Abstract
The urea induced equilibrium denaturation behavior of glutaminyl-tRNA synthetase from Escherichia coli (GlnRS) in 0.25 m potassium l-glutamate, a naturally occurring osmolyte in E. coli, has been studied. Both the native to molten globule and molten globule to unfolded state transitions are shifted significantly toward higher urea concentrations in the presence of l-glutamate, suggesting that l-glutamate has the ability to counteract the denaturing effect of urea. d-Glutamate has a similar effect on the equilibrium denaturation of glutaminyl-tRNA synthetase, indicating that the effect of l-glutamate may not be due to substrate-like binding to the native state. The activation energy of unfolding is not significantly affected in the presence of 0.25 m potassium l-glutamate, indicating that the native state is not preferentially stabilized by the osmolyte. Dramatic increase of coefficient of urea concentration dependence (m) values of both the transitions in the presence of glutamate suggests destabilization and increased solvent exposure of the denatured states. Four other osmolytes, sorbitol, trimethylamine oxide, inositol, and triethylene glycol, show either a modest effect or no effect on native to molten globule transition of glutaminyl-tRNA synthetase. However, glycine betaine significantly shifts the transition to higher urea concentrations. The effect of these osmolytes on other proteins is mixed. For example, glycine betaine counteracts urea denaturation of tubulin but promotes denaturation of S228N λ-repressor and carbonic anhydrase. Osmolyte counteraction of urea denaturation depends on osmolyte-protein pair.
Item Type: | Article |
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Source: | Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology. |
ID Code: | 43126 |
Deposited On: | 10 Jun 2011 05:48 |
Last Modified: | 18 May 2016 00:13 |
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