Progesterone receptor expression in the human placenta

Gopi Shanker, Y. ; Jagannadha Rao, A. (1999) Progesterone receptor expression in the human placenta Molecular Human Reproduction, 5 (5). pp. 481-486. ISSN 1360-9947

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The presence of progesterone receptors (PR) in the human placenta has been demonstrated using the reverse transcriptase-polymerase chain reaction technique. It was observed that the amount of PR in the human placenta is less during late gestation. Electrophoretic mobility shift assays with nuclear extract isolated from the first trimester and term placenta revealed three complexes when incubated with [32P]dCTP-labelled progesterone response element, and, in competition with unlabelled progesterone response element, the formation of all three complexes was inhibited. When supershift analysis of these complexes was carried out using antibodies which cross-react with both the A and B types of the PR or only with the B type receptor, only the A-form of PR was detected in the human placenta.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
Keywords:Electrophoretic Mobility Shift Assay; Placenta; PR Monoclonal Antibody Progesterone Receptor; RT-PCR
ID Code:43042
Deposited On:09 Jun 2011 09:23
Last Modified:18 May 2016 00:08

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