Rao, Manchanahalli S. ; Wu, Benjamin C. ; Waxman, Jeffrey ; Busch, Harris (1975) Rigid structural requirement of the 5' terminus of mRNA for translational activity Biochemical and Biophysical Research Communications, 66 (4). pp. 1186-1193. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(75)90484-2
Abstract
Oxidation by sodium periodate of the ribose cis-diol of the 5' terminal of liver mRNA to the corresponding dialdehyde virtually destroyed its template activity in the wheat germ translation system. The rigid structural requirement for the ribose cis-diol is indicated by the failure of reduction of the dialdehyde to the corresponding primary alcohols to restore the template activity of the mRNA. Sodium periodate alone inhibited the translational system at concentrations above 0.25 mM. Purification of the periodate oxidized mRNA by sucrose density gradient centrifugation or exclusion on Sephadex G-100 did not increase its template activity. Periodate oxidized mRNA was not inhibitory to translation of untreated mRNA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 42509 |
| Deposited On: | 04 Jun 2011 10:12 |
| Last Modified: | 04 Jun 2011 10:12 |
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