Aggarwal, Rakesh ; McCaustland, Karen A. (1998) Hepatitis E virus RNA detection in serum and feces specimens with the use of microspin columns Journal of Virological Methods, 74 (2). pp. 209-213. ISSN 0166-0934
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01660...
Related URL: http://dx.doi.org/10.1016/S0166-0934(98)00049-4
Abstract
This report describes the use of microspin columns for extraction of hepatitis E virus (HEV) RNA from stool and serum specimens for reverse transcription-polymerase chain reaction (RT-PCR) and compares this method with the glass powder method. The microspin column method was found to be 1- to 2-log more sensitive in detecting HEV RNA than the glass powder method and had better reproducibility. The microspin column method also detected HEV RNA in a larger number of specimens than the glass powder method from among a panel of serum and stool specimens. Use of this method may allow better assessment of viremia and fecal excretion in patients and experimental animals infected with HEV.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Hepatitis E; Hepatitis E Virus; Polymerase Chain Reaction; Viral Excretion |
| ID Code: | 4035 |
| Deposited On: | 13 Oct 2010 06:58 |
| Last Modified: | 31 Dec 2010 04:52 |
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