Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

Venugopal, T. ; Mathavan, S. ; Pandian, T. J. (2002) Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy Journal of Biosciences, 27 (3). pp. 261-272. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jbiosci/jun2002/261.pdf

Related URL: http://dx.doi.org/10.1007/BF02704915

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

Item Type:	Article
Source:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	cDNA Cloning; Growth Hormone; Indian Major Carps; Overexpression; Protein 3-D Structure; RACE
ID Code:	39833
Deposited On:	17 May 2011 11:50
Last Modified:	17 May 2016 22:07

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