Prasad, Rajendra ; HÖfer, Milan (1986) Tetraphenylphosphonium is an indicator of negative membrane potential in Candida albicans Biochimica et Biophysica Acta (BBA) - Biomembranes, 861 . pp. 377-380. ISSN 0005-2736
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...
Related URL: http://dx.doi.org/10.1016/0005-2736(86)90442-6
Abstract
The characteristics of the uptake of lipophilic cations tetraphenylphosphonium (TPP+) into Candida albicans have been investigated to establish whether TPP+ can be used as a membrane potential probe for this yeast. A membrane potential (Δψ, negative inside) across the plasma membrane of C. albicans was indicated by the intracellular accumulation of TPP+. The steady-state distribution of TPP+ was reached within 60 min and varied according to the expected changes of Δψ. Agents known to depolarize membrane potential caused a rapid and complete efflux of accumulated TPP+. The initial influx of TPP+ was linear over a wide range of TPP+ concentrations (2.5-600 µM), indicating a non mediated uptake. Thus, TPP+ is a suitable Δψ probe for this yeast.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Tetraphenylphosphonium Ion; Membrane Potential; (C. albicans) |
| ID Code: | 39404 |
| Deposited On: | 12 May 2011 08:20 |
| Last Modified: | 12 May 2011 08:20 |
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