Sharma, Arun Kumar ; Sharma, Archana (1957) Permanent smears of leaf tips for the study of chromosomes Biotechnic & Histochemistry, 32 (4). pp. 167-169. ISSN 1052-0295
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Official URL: http://informahealthcare.com/doi/abs/10.3109/10520...
Abstract
Young leaf tips are soaked in a saturated aqueous esculin (aesculine) solution at 10-12°C for 15 min to 24 hr and fixed in acetic-alcohol, 1:1. The materials are then stained in a mixture of 2% aceto-orcein and 12V HCl (9:1), 3-4 sec over a flame followed by 30 min or longer at 30°C and then smeared in 1% aceto-orcein. Preparations are made permanent by loosening the cover glass in tertiary butyl alcohol and mounting directly in Canada balsam.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Informa plc.. |
| ID Code: | 39047 |
| Deposited On: | 06 May 2011 10:10 |
| Last Modified: | 06 May 2011 10:10 |
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