Peptide synthesis catalysed by papain immobilised on polymer supports: effect of the macromolecular structure and reaction conditions on synthesis

Jayakumari, V. G. ; Rajasekharan Pillai, V. N. (1991) Peptide synthesis catalysed by papain immobilised on polymer supports: effect of the macromolecular structure and reaction conditions on synthesis Proceedings of the Indian Academy of Sciences - Chemical Sciences, 103 (2). pp. 133-148. ISSN 0253-4134

[img]
Preview
PDF - Publisher Version
1MB

Official URL: http://www.ias.ac.in/j_archive/chemsci/103/3/133-1...

Related URL: http://dx.doi.org/10.1007/BF02843563

Abstract

Papain immobilised on different types of polymeric supports was used for the synthesis of peptides in aqueous-organic solvent mixtures. The effects of the nature of the polymer support, degree of crosslinking, nature and length of the spacer grouping between the polymer backbone and the point of attachment of the enzyme, and reaction conditions like pH, concentration of nucleophile and the immobilised enzyme content on the course of the synthesis were investigated. Divinylbenzene-crosslinked polystyrene, divinylbenzenecrosslinked polyacrylamide and N,N'-methylene-bis-acrylamide-crosslinked polyacrylamide systems immobilised with papain were used for these studies. An increase in the length of the spacer arm and an increase in hydrophilicity invariably resulted in an increase in the yield of the peptide synthesis. Papain immobilised on polystyrene-PEG supports and tetraethyleneglycol-crosslinked polystyrene supports was determined to be more efficient in effecting peptide synthesis when compared to other polystyrene-based supports.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Immobilised Enzymes; Enzymatic Peptide Synthesis; Immobilised Papain; Functionalised Polymeric Supports
ID Code:38614
Deposited On:23 May 2011 06:20
Last Modified:17 May 2016 21:24

Repository Staff Only: item control page