Roice, M. ; Suma, G. ; Kumar, K. S. ; Rajasekharan Pillai, V. N. (2001) Synthesis of esculentin-1 antibacterial peptide fragments on 1,4-butanediol dimethacrylate cross-linked polystyrene support Journal of Protein Chemistry, 20 (1). pp. 25-32. ISSN 0277-8033
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Official URL: http://www.springerlink.com/content/gm014n404j2742...
Related URL: http://dx.doi.org/10.1023/A:1011048919748
Abstract
Peptide segments corresponding to antibacterial esculentin-1 (1–15), (33–44), (9–27), and their modified forms were synthesized on 1,4-butanediol dimethacrylate cross-linked polystyrene (PS-BDODMA) support. Hydroxymethyl and aminomethyl 2% PS-BDODMA supports were used for the synthesis. The HMPB linker was appended to the aminomethyl resin using HBTU in presence of HOBt and the first amino acid was incorporated using MSNT. The conventional Fmoc synthetic protocol was used for the synthesis of peptides. The peptides were cleaved from the support using TFA. The peptides were purified by HPLC, and characterized by amino acid analysis and MALDI TOF MS. The secondary structures of the peptides were revealed by CD measurements. The synthesis of these peptides illustrates the utility of the new support for the synthesis of long-chain bioactive peptides. The synthetic peptides were tested for antimicrobial activity against Escherichia coli Mos blue, E. coli 2, Bacillus brevis, B. megaterium, Pseudomonas HTL, and Vibrio mimicus. The antibacterial activity of the peptides was explained on the basis of the helicity and charged nature of the sequences.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer. |
Keywords: | Esculentin-1 and Analogues; SPPS; Antibacterial Activity; PS-BDODMA Resin |
ID Code: | 38571 |
Deposited On: | 23 May 2011 07:58 |
Last Modified: | 23 May 2011 07:58 |
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