Self-stimulation rewarding experience induced alterations in dendritic spine density in CA3 hippocampal and layer V motor cortical pyramidal neurons

Shankaranarayana Rao, B. S. ; Raju, T. R. ; Meti, B. L. (1999) Self-stimulation rewarding experience induced alterations in dendritic spine density in CA3 hippocampal and layer V motor cortical pyramidal neurons Neuroscience, 89 (4). pp. 1067-1077. ISSN 0306-4522

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/s0306-...

Related URL: http://dx.doi.org/10.1016/S0306-4522(98)00394-7

Abstract

Self-stimulation rewarding experience induced alterations in the numerical density of spines in CA3 hippocampal and layer V motor cortical pyramidal neurons in adult male Wistar rats was evaluated. Self-stimulation experience was provided 1 h daily over a period of 10 days through stereotaxically implanted bipolar stainless steel electrodes bilaterally in lateral hypothalamus and substantia nigra-ventral tegmental area. After 10 days, rats were killed and the hippocampus and motor cortex were processed for rapid Golgi staining procedure. The dendritic spine densities were studied in CA3 hippocampal and layer V motor cortical pyramidal neurons. The spine densities were quantified in five successive segments of 15.2 μm up to a distance of 76 μm. Apical dendrites were classified as mainshaft, sub branch, oblique shaft-I, oblique shaft-II, primary branch; and basal dendrites as main shaft, primary branch and secondary branch. A grand total of 864 CA3 hippocampal and 1 008 layer V motor cortical dendrites were analysed for spine counting in different groups of rats. The results revealed a significant (P<0.001; ANOVA, F-test) increase in the number of spines in all the categories of dendrites in apical and basal regions in both hippocampal and motor cortical neurons in self-stimulation group of rats. Such changes were not observed either in sham control, experimenter-administered or normal control groups of rats. The self-stimulation induced increase in the spine density suggests an increase in the postsynaptic receptive field in CA3 hippocampal and layer V motor cortical neurons. This might enhance the efficacy of synaptic transmission in these neurons. Our study clearly demonstrated the self-stimulation rewarding experience induced postsynaptic plasticity in hippocampal and motor cortical pyramidal neurons.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Self-stimulation Reward; Hippocampal; CA3 Neurons; Motor Cortex; Layer V Pyramidal Neurons; Dendritic Spines; Postsynaptic Plasticity
ID Code:38494
Deposited On:22 Nov 2011 04:27
Last Modified:22 Nov 2011 04:27

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