Cryptic blues: mechanism in sight!

Abstract

Several plant responses are mediated by blue-light as also UV-A radiation. Until a few years ago, the identity of the blue/UV-A sensing photoreceptors was equivocal. In the past few years, however, the molecular genetic analysis of Arabidopsis mutants has greatly aided in the identification and characterization of blue/UV-A sensing cryptochrome 1 (cry1), cryptochrome 2 (cry2) and phototropin (JK224/ nph1). The cry1 and cry2 receptors, like DNA photolyases, are dual chromophore proteins and may harbour both a flavin (FAD) and a pterin for primarily regulating hypocotyl growth inhibition, cotyledon expansion and flowering time, besides sensing light for entraining endogenous clocks. In contrast, JK224/nph1 anchors another flavin, FMN, and regulates phototropism. In addition to flavins and pterin, evidence is mounting in favour of a carotenoid, zeaxanthin, for regulating blue-light-induced stomatal opening. There is little information on the signalling components acting downstream to blue sensory receptors, but nph1 has an intrinsic kinase domain that on photoexcitation initiates a phosphorelay through interaction with phosphoproteins like NPH3. Although the primary mechanism of action of cry1 and cry2 is unknown, their localization in the nucleus and protein-protein interaction with phytochromes indicates that they may directly regulate changes in gene expression. The occurrence of cryptochromes in both plants and animals indicates their ubiquitous nature and a prominent role in regulating diverse responses.