The nucleus of the living zygote of Saccharomyces carlsbergensis

Abstract

1. The zygotes ofSaccharomyces carlsbergensis retain the shape assumed at the time of their origin by the fusion of two spores even after they have produced a few buds. 2. The nucleus is visible in the zygotes on the 7th day after the introduction of spores into fresh wort. A nuclear membrane delimits the nucleus from the cytoplasm and encloses formed structures. The structure of the nucleus is identical in a zygote and its bud. 3. Iodine-formaldehyde-acetic gives a life-like preservation of cell structures. After removal of the cytoplasmic basophilia by acid hydrolysis, staining with hæmatoxylin gives pictures of nuclear details comparable to that of the living nucleus. The Feulgen stained area is only a portion of the resting nucleus.