Suresh Kumar, T. K. ; Raman, B ; Mohan Rao, Ch. (1995) Fluorescent staining for proteins on polyacrylamide gels with 5-dimethylamino-1-naphthalenesulfonyl chloride (dansyl chloride) Journal of Biochemical and Biophysical Methods, 30 (1). pp. 79-84. ISSN 0165-022X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/016502...
Related URL: http://dx.doi.org/10.1016/0165-022X(94)00064-K
Abstract
A simple and sensitive post electrophoresis fluorescent staining technique for proteins on polyacrylamide gels using 5-dimethylamino-1-naphthalene sulfonyl chloride (dansyl chloride) has been developed. Dansyl chloride staining increases the sensitivity, 0.125 μg protein per band can be visualised by this technique. The staining method appears to be applicable to all types of proteins including proteoglycans.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Fluorescence; Stain; PAGE; Electrophoresis; Protein; Dansyl Chloride; Gel |
| ID Code: | 36869 |
| Deposited On: | 16 Apr 2011 13:52 |
| Last Modified: | 29 Nov 2011 10:00 |
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