Trivedi, Vishwa D. ; Raman, Bakthisaran ; Ramakrishna, Tangirala ; Mohan Rao, Ch. (1999) Detection and assay of proteases using calf lens β-crystallin aggregate as substrate Journal of Biochemical and Biophysical Methods, 40 (1-2). pp. 49-55. ISSN 0165-022X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01650...
Related URL: http://dx.doi.org/10.1016/S0165-022X(99)00021-4
Abstract
The eye lens protein, βL-crystallin, aggregates and yields a turbid solution upon refolding from its denatured state. We have observed that the addition of trace amounts of protease results in clearing of this turbidity. Based on this observation, we have developed a simple and rapid method for the detection and assay of proteases. This assay can be performed in the pH range of 6.0–9.0. We could assay the activity of trypsin at a concentration as low as 5 μg/ml.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Protease Detection; Turbidity Clearance |
| ID Code: | 36841 |
| Deposited On: | 16 Apr 2011 14:02 |
| Last Modified: | 29 Nov 2011 10:02 |
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