Berrya, S. F. ; Patnaik, G. ; Cocking, E. C. ; Pental, D. (1983) Plant regeneration from protoplasts of Nicotiana alata 'Crimson Bedder' for somatic hybridisation studies Scientia Horticulturae, 20 (3). pp. 281-285. ISSN 0304-4238
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030442...
Related URL: http://dx.doi.org/10.1016/0304-4238(83)90009-2
Abstract
Protoplasts could be isolated readily from mesophyll and suspension cells of Nicotiana alata cultivar 'Crimson Bedder' and could be cultured in liquid media to yield small colonies. Suspension culture protoplasts exhibited a higher plating efficiency, and colonies regenerated from mesophyll protoplasts possessed enhanced plant regeneration capacity. These protoplast systems could be used for somatic hybridisation assessment with Petunia using a simple procedure for manual isolation and culture of heterokaryons.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | In Vitro; Nicotiana alata; Petunia; Protoplasts; Regeneration; Somatic Hybridisation |
| ID Code: | 36671 |
| Deposited On: | 11 Apr 2011 14:28 |
| Last Modified: | 11 Apr 2011 14:28 |
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