Mukhopadhyay, Arundhati ; Töpfer, Reinhard ; Pradhan, Akshay K. ; Sodhi, Yaspal S. ; Steinbiß, Hans-Henning ; Schell, Jeff ; Pental, Deepak (1991) Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake Plant Cell Reports, 10 (8). pp. 375-379. ISSN 0721-7714
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Official URL: http://www.springerlink.com/content/836e983f4aacba...
Related URL: http://dx.doi.org/10.1007/BF00232604
Abstract
Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors carrying marker genes for resistance to methotrexate (dhfr), hygromycin (hpt) and phosphinotricin (bar) were constructed and used for transformation. Large number of normal, fertile transformants were obtained with vectors carrying hpt and bar genes. No transformants could be regenerated for resistance to methotrexate as it severely suppressed shoot differentiation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Brassica oleracea; Direct Gene Transfer; Protoplast Regeneration; Transformation |
| ID Code: | 36277 |
| Deposited On: | 11 Apr 2011 14:34 |
| Last Modified: | 11 Apr 2011 14:34 |
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