Folin-Ciocalteu reagent for the estimation of siderochromes

Subramanian, K. N. ; Padmanaban, G. ; Sarma, P. S. (1965) Folin-Ciocalteu reagent for the estimation of siderochromes Analytical Biochemistry, 12 (1). pp. 106-112. ISSN 0003-2697

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...

Related URL: http://dx.doi.org/10.1016/0003-2697(65)90147-8

Abstract

1. 1. A simple method has been devised for the estimation of siderochromes based on their reaction with Folin-Ciocalteu reagent to give a blue complex under alkaline conditions. 2. 2. The applicability of the method to biological systems has been tested with N. crassa and concentrations in the ranges 5-50 μg and 1-10 μg can be accurately estimated with an over-all recovery of 95%.

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