Kumar, Ashwani ; Padmanaban, G. (1980) Studies on the synthesis of cytochrome P-450 and cytochrome P-448 in rat liver Journal of Biological Chemistry, 255 (2). pp. 522-525. ISSN 0021-9258
![]()
|
PDF
- Publisher Version
475kB |
Official URL: http://www.jbc.org/content/255/2/522.short
Abstract
The synthesis of cytochrome P-450 (phenobarbital inducible) and cytochrome P-448 (3-methylcholanthrene inducible) have been studied in rat liver in vivo and in the wheat germ cell-free system using anti-cytochrome P-450 and anti-cytochrome P-448 antibodies. The major mature forms synthesized in vivo correspond to a molecular weight of 47,000 for cytochrome P-450 and 53,000 for cytochrome P-448. Translation of poly(A)-containing RNA from phenobarbital-treated rats in the wheat germ cell-free system reveals that the cell-free product immunoprecipitated with anti-cytochrome P-450 antibody has a molecular weight close to 47,000. In the case of 3-methylcholanthrene, the cell-free product immunoprecipitated with anti-cytochrome P-448 antibody shows a molecular weight around 59,000. Significant conversion of the 59,000 species to the 53,000 species can be demonstrated when the translation is carried out in the presence of microsomal membranes isolated from rat liver. Phenobarbital and 3-methylcholanthrene enhance the translatable messenger RNA contents for cytochrome P-450 and cytochrome P-448, respectively.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to American Society for Biochemistry and Molecular Biology. |
ID Code: | 34381 |
Deposited On: | 18 Apr 2011 14:00 |
Last Modified: | 17 May 2016 17:16 |
Repository Staff Only: item control page