Effect of gibberellic acid and salicylic acid on the activity and electrophoretic pattern of IAA-oxidase during floral induction in Impatiens balsamina

Abstract

IAA-oxidase activity increased in the stem as well as in the leaves of plants treated with GA₃, SA and GA₃ + SA during the early stages under inductive and non-inductive photoperiods, the activity being the highest in GA₃ + SA-treated plants. An isoenzyme of IAA-oxidase with Rm 0.15 developed in the stem as well as in the leaves subsequent to 1 or 2 inductive treatments. As this band persisted till the end of the experiment, it may be associated with the initiation as well as development of floral buds. Another band (Rm 0.30) appears to be associated with the phenol (SA) as it developed in the stem as well as in the leaves of SA- and GA₃ + SA-treated plants under both photoperiods. A band with Rm 0.60 developed in the leaves but not in the stem of GA₃-, SA- and GA₃ + SA-treated plants under both photoperiods.