Estabrook, R. W. ; Maitra, P. K. (1964) A fluorometric method for the enzymic determination of glycolytic intermediates Analytical Biochemistry, 7 (4). pp. 472-484. ISSN 0003-2697
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(64)90156-3
Abstract
A fluorometric method has been described that allows the microdetermination of glycolytic intermediates in extracts from tissues or celis. The method utilizes commercially available crystalline enzymes of glycolysis coupled finally to a pyridine nucleotide-linked dehydrogenase so that the time course of the reaction is automatically recorded. Illustrations have been presented showing the applicability of the method to the quantitative assay of intermediates down to a fraction of a micromolar range of concentration in the assay system.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 31267 |
| Deposited On: | 25 Apr 2011 09:25 |
| Last Modified: | 09 Jun 2011 09:23 |
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