Estabrook, Ronald W. ; Maitra, P. K. (1962) A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides Analytical Biochemistry , 3 (5). pp. 369-382. ISSN 0003-2697
Full text not available from this repository.
Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(62)90065-9
Abstract
The present study describes the application of a commercial fluorimeter to the enzymic assay of pyridine and adenine nucleotides in neutralized extracts of cellular material. The method retains the advantage of recording the reaction kinetics during the assay with the increased sensitivity associated with the fluorimetric method. The routine application of the method to the detection of 0.1 to 1.0 µmolar concentrations of nucleotides in extracts of yeast cells, liver mitochondria, and submitochondrial particles has been illustrated. In addition, various facets of the assay procedure have been discussed as well as problems associated with the determination of optimal conditions for the application of protein precipitants to terminate the reaction.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 31263 |
| Deposited On: | 25 Apr 2011 09:24 |
| Last Modified: | 27 Apr 2011 06:30 |
Repository Staff Only: item control page
