Serodiagnosis of leishmaniasis with recombinant ORFF antigen

Raj, V. S. ; Ghosh, A. ; Dole, V. S. ; Madhubala, R. ; Myler, P. J. ; Stuart, K. D. (1999) Serodiagnosis of leishmaniasis with recombinant ORFF antigen The American Journal of Tropical Medicine and Hygiene, 61 (3). pp. 482-487. ISSN 0002-9637

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The serodiagnostic potential of recombinant ORFF protein (rORFF) from Leishmania infantum was assessed by ELISA. Of 49 sera from confirmed cases of visceral leishmaniasis (VL), all were seropositive using 5 ng of rORFF and serum diluted 1:20, while only 38 were positive with 500 ng of soluble antigen (SA) and 44 were positive by a direct agglutination test. There was also a positive correlation between spleen size and level of seropositivity with rORFF or SA. The reciprocal endpoint titer with rORFF was 1,280 for sera from VL patients, but < 20 with sera from malaria, filariasis, and tuberculosis patients, as well as with sera from healthy individuals from endemic and non-endemic areas. Sera from 10 confirmed cutaneous leishmaniasis cases from Turkey were negative or only weakly positive with rORFF although 9 were positive with SA. Thus, rORFF protein appears useful as a sensitive reagent for the differential diagnosis of VL caused by the Leishmania donovani complex.

Item Type:Article
Source:Copyright of this article belongs to American Society of Tropical Medicine and Hygiene.
ID Code:29838
Deposited On:23 Dec 2010 04:55
Last Modified:17 May 2016 12:37

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