Shenoy, M. A. ; Mishra, K. P. ; Singh, B. B. ; Gopal-Ayengar, A. R. (1974) A study of reactions of N-ethyl malaeimide with cellular fractions during radiosensitization of E. coli B/R International Journal of Radiation Biology, 25 (3). pp. 303-311. ISSN 0955-3002
Full text not available from this repository.
Official URL: http://informahealthcare.com/doi/abs/10.1080/09553...
Related URL: http://dx.doi.org/10.1080/09553007414550351
Abstract
E. coli B/r cells γ-irradiated under anoxic conditions and in the presence of NEM showed enhanced damage in terms of their colony-forming ability. Irradiation leads to binding of NEM with cellular macromolecules, particularly proteins. Binding occurs to all amino acid residues. In vitro irradiation of bovine serum albumin in aqueous solution has confirmed this. A reaction between NEM and amino acid radical has been implicated. The binding of NEM with cellular proteins may lead to inactivation of enzymes involved in post-irradiation biochemical processes giving rise to enhanced damage. NEM also reacts with amino acids in the absence of radiation, which may partly explain the residual sensitization noticed in cells treated with NEM. Part of the sensitizing effect of NEM has been shown to be due to its electron affinic property.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to Informa Healthcare. |
ID Code: | 29501 |
Deposited On: | 18 Dec 2010 05:27 |
Last Modified: | 02 Jun 2011 05:01 |
Repository Staff Only: item control page