Raizada, M. K. ; Krishna Murti, C. R. (1972) Synthesis of RNA, protein, cellulose, and mucopolysaccharide and changes in the chemical composition of Hartmannella culbertsoni during encystment under axenic conditions Journal of Eukaryotic Microbiology, 19 (4). pp. 691-695. ISSN 1066-5234
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1550-...
Related URL: http://dx.doi.org/10.1111/j.1550-7408.1972.tb03562.x
Abstract
Hartmannella culbertsoni trophozoites are transformed into viable cysts on exposure to a non-nutrient agar medium containing 15 mM MgCl2 and 20 mM taurine. Amebae differentiating in this encystment medium incorporate more uracil-2-14C into RNA and more leucine-1-14C or valine-1-14C into proteins than controls. Encysting organisms incorporate significantly more glucose-U-14C into cellulose and glucosamine-1-14C into mucopolysaccharides. Incorporation of glucose-U-14C into cellulose and of glucosamine-1-14C into mucopolysaccharides are inhibited by actinomycin D or cycloheximide.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Society of Protozoologists. |
| Keywords: | Hartmannella culbertsoni; Encystment; Encystment Medium; Incorporation of 14C-labelled Compounds; Rna; Proteins; Cellulose; Mucopolysaccharides; Actinomycin D and Cycloheximine Inhibition |
| ID Code: | 29374 |
| Deposited On: | 17 Dec 2010 08:09 |
| Last Modified: | 04 Jun 2011 09:37 |
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