Bhattacharya, Alok ; Bhattacharya, Sudha ; Sharma, M. P. ; Diamond, Louis S. (1990) Metabolic labeling of entamoeba histolytica antigens: characterization of a 28-kDa major intracellular antigen Experimental Parasitology, 70 (3). pp. 255-263. ISSN 0014-4894
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001448...
Related URL: http://dx.doi.org/10.1016/0014-4894(90)90107-N
Abstract
The in vivo incorporation of radiolabeled amino acids into antigens of Entamoeba histolytica, HM-1,IMSS, is reported. Immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kDa antigen present in whole cell lysates of E. histolytica. This antigen was of cytoplasmic origin, as indicated by cell fractionation and Triton X-114 detergent-phase separation. Immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kDa antigen as the major antigen recognized by the sera tested. Immunoprecipitation analysis using radiolabeled-released proteins instead of whole cell lysates showed a number of bands, including the 28-kDa antigen. The data suggest that the 28-kDa antigen is of cytoplasmic origin or is released from the cytoplasmic compartment.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Entamoeba histolytica; Metabolic Labeling; Antigenic Analysis; Secretory Proteins; Triton X-114 Detergent Phase Extraction |
| ID Code: | 2857 |
| Deposited On: | 09 Oct 2010 05:41 |
| Last Modified: | 24 Jan 2011 06:13 |
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