Transient suppression of F-plasmid incompatibility in a strain of Escherichia coli

Abstract

The incompatibility between F plasmids is transiently suppressed in Escherichia coli strain CSH54. As a result this strain is able to maintain two F^' factors or an F^' factor and a mini-F plasmid for considerably longer periods than normal strains. When selective pressure for two markers carried by two separate F^'s (or an F^' and mini-F) is imposed on normal strains, the two plasmids tend to form a cointegrate structure which can be detected genetically by the joint transfer of both the markers upon mating. This does not happen in CSH54; instead, the two plasmids are maintained and transferred independently. Physical evidence for the maintenance of an F^' and a mini-F plasmid is provided by agarose gel electrophoresis.